Unico 2800 uv vis спектрофотометр руководство по эксплуатации

Однолучевой спектрофотометр UNICO модель 2800.

Работающий в спектральном диапазоне 190-1100 нм, является самой экономичной разработкой в серии однолучевых приборов UNICO 280X. Эта модель, имеющая спектральную щель 4 нм и скорость сканирования до 1000 нм/мин специально адаптирована для отечественных условий и выпускается с учетом российских лабораторных требований. Прибор отличается высокой точностью определения процента пропускания/ оптической плотности, установки длины волны, разрешением 0,1 нм и прекрасной временной стабильностью.

Спектрофотометр UNICO 2800 является настольным прибором, оснащенным большим информативным жидкокристаллическим экраном.(320х240 точек), 29-знаковой клавиатурой, встроенным мощным процессором, позволяющим проводить все аналитические измерения без подключения к персональному компьютеру. Режимы измерений, градуировки и полученные спектры хранятся в энергонезависимой памяти. Для вывода информации предусмотрены порты для подключения принтера и компьютера. Для удобства хранения и обработки данных на персональном компьютере разработано специальное программное обеспечение, обеспечивающее перенос данных, составление отчетов, представление результатов измерений.

Вместительное отделение для проб позволяет размещать кюветы длиной до 100 мм. Предлагается широкий ряд держателей проб/кювет и дополнительных принадлежностей. Возможна установка кассеты для программируемой автоматической смены 8-ми квадратных кювет 10х10 мм. Для медико-биологических измерений особенно интересна проточная терморегулируемая (15-40^оС) система с собственным перистальтическим насосом и программатором температуры на Пельтье-элементах.

Конструкция спектрофотометров UNICO серии 280Х основана на оригинальных оптических элементах с защитным покрытием, смонтированных на мощной плите. Источниками излучения служат дейтериевая и галогеновая лампы, доступные для самостоятельной смены и юстировки. Программный счетчик времени работы ламп позволяет планировать их эксплуатацию и своевременную замену.

Метрологическая поддержка.

На основании положительных результатов испытаний спектрофотометр UNICO модель 2800 внесен как тип в государственный реестр средств измерений РФ и зарегистрирован под № 54737-13

Спектрофотометры UNICO 2800 имеют описание типа средств измерений, снабжаются свидетельством о госпроверке, методикой поверки, руководством по эксплуатации на русском языке.

Приборы подлежат ежегодной поверке силами ЦСМ на местах с использованием стандартных поверочных средств.

Технические характеристики

Стандартный комплект поставки

• Спектрофотометр — 1
• Шнур питания — 1
• Чехол от пыли — 1
• Лампа галогеновая запасная — 1
• 4-х позиционный держатель для кювет 10х10 мм — 1
• 3-х позиционный держатель для кювет 10-100мм*24 мм — 1
• Кювета кварцевая 10х10 мм — 2
• Кювета стеклянная 10х10 мм — 4
• Руководство по эксплуатации на русском языке — 1
• Сертификат — 1
• Паспорт — 1
• Методика поверки — 1
• Свидетельство о первичной поверке (либо клеймо поверителя в паспорте) 1

Дополнительные принадлежности (поставляются по заказу).

• Держатель для цилиндрических кювет 8-20 мм.
• 4-х позиционный держатель для кювет 10х100 мм.
• Держатель кюветы 10х10 мм с водяной рубашкой
• Микрокювета для проб до 10 мкл.
• Держатель для измерения отражательной способности
• 8-позиционный автоматический держатель — сменщик кювет
• Контроллер температуры на Пельте-элементах
• Перистальтический насос с проточной кюветой
• Кюветы различных размеров и материалов.
• Пакет обработки и передачи данных (ПО) — Позволяет обрабатывать данные, полученные на автономных сканирующих спектрофотометрах серии SpectroQuest, мод. 2800 – 2804. Поставляется на CD в комплекте с электронным ключом в USB-порт компьютера, описание на русском языке.

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Manuals and User Guides for Unico UV-2800. We have 1 Unico UV-2800 manual available for free PDF download: Service Manual

• 1

  Contents

  Safety . 2

  G e n e r a l . 2

  E l e c t r i c a l . . 2

  Warning . . 2

  P e r f o r m a n c e 3

  Radio Interference . . 3

  I n t r o d u c t i o n 3

  W o r k i n g P r i n c i p l e . . 4

  Unpack ing Ins t ruc t ions . 4

  Specifications .. 5

  Installation . 5

  Operation .. 6

  Prepare the Spectrophotometer 6

  Description of keys 6

  Turn on spectrophotometer . . 7

  Basic operation . 8

  Analyse Sample 12

  Basic Mode . . 12

  Quantitative . . 15

  WL Scan . . . 20

  Kinetics . 2 5

  DNA/Protein 28

  Multi Wavelength .. 31

  Set t ing and Cal ibra t ion . 33

  Utility 3 3

  Defined Tests . . . 4 3

  Appendix A . . 4 6

  A p p e n d i x B 47

  A p p e n d i x C 54

• 2

  Safety: The safety statements in this manual comply with the
  requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the
  following before installing and using the instrument and its
  accessories. The UNICO UV-2800 should be operated by appropriate
  laboratory technicians. General: The apparatus described in this
  manual is designed to be used by properly trained personnel in a
  suitable equipped laboratory. For the correct and safe use of this
  apparatus it is essential that laboratory personnel follow
  generally accepted safe procedures in addition to the safety
  precautions called for in this manual. The covers on this
  instrument may be removed for servicing. However, the inside of the
  power supply unit is a hazardous area and its cover should not be
  removed under any circumstances. There are no serviceable
  components inside this power supply unit. For UNICO UV-2800, avoid
  touching the high voltage power supply at all times. Some of the
  chemicals used in spectrophotometry are corrosive and/or
  inflammable and samples may be radioactive, toxic, or potentially
  infective. Care should be taken to follow the normal laboratory
  procedures for handling chemicals and samples. Electrical: Before
  switching on the apparatus, make sure it is set to the voltage of
  the local power supply (see Installation). The power cord shall be
  inserted in a socket provided with a protective earth contact. The
  protective action must not be negated by the use of an extension
  cord without a protective conductor. Warning: Any interruption of
  the protective conductor inside or outside the apparatus or
  disconnection of the protective earth terminal is likely to make
  the apparatus dangerous. Intentional interruption is prohibited.
  Whenever it is likely that the protection has been impaired, the
  apparatus shall be made inoperative and be secured against any
  unintended operation. NEVER touch or handle the power supply on
  UNICO UV-2800 due to the high voltage. The protection is likely to
  be impaired if, for example, the apparatus Shows visible damage
  Fails to perform the intended measurements Has been subjected to
  prolonged storage under unfavorable conditions Has been subjected
  to severe transport stresses

• 3

  Performance: To ensure that the instrument is working within its
  specification, especially when making measurements of an important
  nature, carry out performance checks with particular reference to
  wavelength and absorbance accuracy. Performance checks are detailed
  in this manual. Radio Interference: For compliance with the EMC
  standards referred to in the EC Declaration of Conformity, it is
  necessary that only shielded cables supplied by us are used when
  connecting the instrument to computers and accessories.
  Introduction: The UNICO UV-2800 model spectrophotometer (Fig 1) is
  a single beam, general purpose instrument designed to meet the
  needs of the Conventional Laboratory, The UNICO UV-2800 model
  spectrophotometer is ideal for various applications, such as:
  Chemistry, Biochemistry, Petrochemistry, Environmental Protection,
  Food and Beverage Labs, Water and Waste Water Labs and other fields
  of quality control and research. The UNICO UV-2800 model
  spectrophotometer incorporates a 320240 dot matrix LCD display for
  photometric results, easy operation and wavelength range of 190nm
  to 1100nm. This instrument is ideal for measurements in the visible
  and ultraviolet wavelength region of the electromagnetic
  spectrum.

  Sample Compartment Lid

  Keypad

  LCD Display

  Rod

• 4

  PowerSupply Fan

  PowerSocket

  PrinterInterface

  Contrast Adjusterof LCD

  PowerSwitch

  110V/220VSelect Switch

  SerialComm.

  Fig1

  Working Principle: The spectrophotometer consists of five parts:
  1) Halogen or deuterium lamps to supply the light; 2) A
  Monochromator to isolate the wavelength of interest and eliminate
  the unwanted second order radiation; 3) A sample compartment to
  accommodate the sample solution; 4) A detector to receive the
  transmitted light and convert it to an electrical signal; and 5) A
  digital display to indicate absorbance or transmittance. The block
  diagram (Fig 2) below illustrates the relationship between these
  parts. Block diagram for the Spectrophotometer

  Light Mono- Sample Detector Display Source chromator
  Compartment

  Fig 2

  In your spectrophotometer, light from the lamp is focused on the
  entrance slit of the monochromator where the collimating mirror
  directs the beam onto the grating. The grating disperses the light
  beam to produce the spectrum, a portion of which is focused on the
  exit slit of the monochromator by a collimating mirror. From here
  the beam is passed to a sample compartment through one of the
  filters, which helps to eliminate unwanted second order radiation
  from the diffraction grating. Upon leaving the sample compartment,
  the beam is passed to the silicon photodiode detector and causes
  the detector to produce an electrical signal that is displayed on
  the digital display. Unpacking Instructions: Carefully unpack the
  contents and check the materials against the following packing list
  to ensure that you have received everything in good condition.

  100%T 0 A

• 5

  Packing List Description Quantity

  Spectrophotometer…………………………………………………
  1 Mains
  Lead…………………………………………………………….
  1
  Cuvettes…………………………………………………………………
  Set of 4, glass

  …………………………………………………………..
  Set of 2, quartz Dust
  Cover……………………………………………………………..
  1 Manual
  …………………………………………………………………..
  1 Specifications: Wavelength Range: 190-1100nm Spectral Bandpass:
  4nm Wavelength Accuracy: 0.8nm Wavelength Repeatability: 0.05nm
  Stray Radiant Energy: 0.15%@220nm&340nm Photometric Range:
  0-200%T,-0.3-.2.8A Noise:

• 6

  Operation: Prepare the spectrophotometer Fig 3 is the control
  panel. User can perform all operations by pressing the keys and all
  the results and operation information are displayed on the LCD.

  6MNO +/-/.

  9WXYZ 0

  8TUV

  7PQRS

  PRINT 0Abs100%T

  SET ESCSTOP

  ESCSTOP

  LOAD SAVE START ENTER

  CELL

  F1 F2 F3 F4

  1CLEAR

  4GHI

  5JKL

  3DEF

  2ABC

  LCD

  Functionkeys

  Numerickeys

  Controlkeys

  Fig 3

  Description of keys LOAD Load data or curve saved before; SAVE
  Save data or curve; SET Set wavelength; 0Abs/100%T Blank or scan
  the base line; PRINT Print test results or screen START Start
  testing or scanning sample; ESC/STOP Exit to previous screen or
  cancel the operation; ENTER Confirm the inputted data or selected
  item; Go into next

  setup or screen; F1-F4 Function based on the information on the
  screen; 0-9 Input number or letter, consecutively press a numeric
  key

  to select a character; +/-/. Input +,- or dot; CLEAR Clear all
  characters when you are inputting or clear curve

  displays on the screen;

• 7

  , Change x scale; Search point after scan; clear a
  character;

  , Change y scale; Search peak after scan; Scroll items for
  selecting; Change capital/small letter last typed in; Browse the
  items for selection;

  CELL Set cell position. Turn on spectrophotometer Turn on
  spectrophotometer by pressing the Power Switch (IO)(see Fig1). The
  instrument starts to initiate and the steps are as below:

  1.The instrument will check memory first (Fig 4), please wait or
  press any key to skip this step ,after positioning filter,
  auto-cell changer(if installed) and D2/W lamps,the screen display
  as Fig 4A. 15 minutes pass or pressESC, the screen display as Fig
  5,Select No to skip to main menu( Fig 7) and select
  Yes(recommended) to calibrate system (Fig 6).The calibrating
  process include get dark current, searching 656.1nm and check
  energy.After finish the calibration system,go to main menu too (Fig
  7).

  2.If the data in memory has been lost, the instrument will
  directly calibrate system without any choice for you.

  3.If no auto-cell changer installed cell #1 will disappear in
  Fig7

  Fig 4

  D2W

  Wait until EasyRTOS booted:Check memory . . . . . . . .Init
  Printer . . . . . . . . . . .Init Comm Port . . . . . . . .Start
  Kernel . . . . . . . . . .Positioning . . . . . . . . . .Warm up 15
  minutes . . .

  Press ESC to skip …

  WL : 656.1nm 16:20:05

  Fig 4A

  United Products & Instruments Inc.

  D2W

  Wait until EasyRTOS booted:Check memory . . . . . . . .

  WL : 656.1nm RAM Checked: 16kb

• 8

  D2W

  Wait until EasyRTOS booted:Check memory . . . . . . . .

  WL : 656.1nm 16:20:18

  Init Printer . . . . . . . . . . .Init Comm Port . . . . . . .
  .Start Kernel . . . . . . . . . .Positioning . . . . . . . . .
  .

  System calibrating? No

  Warm up 15 minutes. . . .

  Fig 5

  D2W

  Wait until EasyRTOS booted:Check memory . . . . . . . .

  United Products & Instruments Inc.

  WL : 656.1nm 16:28:03

  Init Printer . . . . . . . . . . .Init Comm Port . . . . . . .
  .Start Kernel . . . . . . . . . .Positioning . . . . . . . . . .
  Init AD Converter . . . . . .Search 656.1nm . . . . . .Check Energy
  . . . . . .

  Fig 6

  WL : 656.1nm 16:31: 35

  D2WCell #1

  123

  87

  4

  56

  Basic mode Quantitative WL scan Kinetics

  DNA/Protein Multi WL Utility Defined test

  United Products & Instruments Inc.

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  Fig 7

  Basic operation Blank

  Push the blank cuvette into the lightpath. Press the key
  0Abs/100%Tfor blanking

  Note:1. If the reference solution is too thick, Energy Low will
  appear following the Blankingon the screen (Fig 8).If Energy too
  Low appears following the Blanking,the test will be paused and
  Warning will appear on the screen.(Fig 9).

  2.If no automatic changer installed cell #1 and Max E will
  disappear in Fig8

• 9

  WL : 656.1nm Energy Low. . .

  BlankingD2WCell #1

  Max E

  F1:Unit F2:Mode F3: F Factor F4: Standard

  Fig 8 NOTE: 1.Blanking is automatic after a wavelength
  change

  DO NOT OPEN SAMPLE COMPARTMENT LID DURING BLANKING.

  2.The dark current dont be taken after power on,if you bypass
  the calibrating system. It is recommended to take the dark current
  after warm up.See page 38.

  Warning …D2WCell #1

  Max E

  F1:Unit F2:Mode F3: F Factor F4: Standard

  WL : 656.1nm Energy too Low. . .

  Fig 9

  Set wavelength (Example: set wavelength in Basic mode)

  PressSET(Fig 10).

  WL : 656.1nm 12: 35: 27

  0.001 AbsD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  Fig 10

• 10

  Use numeric keypad to input wavelength (Fig 11).

  WL : 656.1nm 12: 35: 27

  0.001 AbsD2WCell #1

  Max E

  Please input wl: 450

  Fig 11 Press ENTER to change the wavelength from 656.1nm to

  450.0nm,and then blank; after blanking, the screen displays as
  Fig 12.

  WL : 450.0nm 12: 35: 27

  0.000 AbsD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  Fig 12 Load or delete data or curve (Take the WL scan test For
  example)

  Press 3 in Fig.7 go into WL scan.AfterLOADbeing pressed,the
  first file (ABC.wav)in memory will appear on the bottom line of
  screen .Showed as Fig 13. Press or to browse the files stroed in
  memory. Then if : 1. The key.ENTERbe pressed,the file selected will
  be loaded and

  displays on the screen.Fig 14. Note(1).The file selected must
  match WL scan tests type.if not the

  file type error will appear on the right of top line.
  (2).Different test has different file type.Refer to table 1 on
  page

  12. 2. The key CLEARbe pressed the file selected will be deleted
  by

  selecting Yes.

• 11

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  F1:Setup F2:Mode F3:Search

  0

  %

  T

  1

  20.0

  Fig 13

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  F1:Setup F2:Mode F3:Search

  0

  %

  T

  1

  20.0

  Fig 14

  Table 1 Test File Type

  Quantitative Curve ***.fit Quantitative Test Result ***.qua

  WL Scan ***.wav Kinetics ***.kin

  DNA/Protein ***.dna Multi WL ***.mul

  WL Validity ***. wlv Accu. Validity ***.phv

  Save data or curve (Example: Save curve in WL scan)

  Press the key SAVE in Fig14 to save curve. Name the curve by
  pressing the numeric keypad (Fig 15), press

  the keyENTER to comfirm.

  . Note(1). Pressing numeric key continually to scroll characters
  and pressing , to alter capital letter to miniscule.Table 2 shows
  all characters built in.

  (2) If the name already exists in memory, the warning duplicated
  name, are you sure will appear . Yesfor overwrite andNofor
  Exit.

  (3) The length of filename is less than 4.

• 12

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  Please input File Name:2

  0

  %

  T

  1

  20.0

  Fig 15

  Table 2 key representing key representing key representing 0
  0,+,-,* ,/ 1 1,#,?,:,I 2 2,A,B,C,= 3 3,D,E,F,% 4 4,G,H,I,{ 5
  5,J,K,L,} 6 6,M,N,O,~ 7 7,P,Q,R,S, 8 8,T,U,V, 9 9,W,X,Y,Z +/-/.
  -,.,

  Print test report (For example: Print the report in Basic
  mode,Fig16) Press the keyPRINTto print the report (curve or data
  you have loaded or tested, Fig 17).

  Fig 16

  Fig 17

  Before measurement Make a blank reference solution by filling a
  clean cuvette (or test tube) half

  full with distilled or de-ionized water or other specified
  solvent. Wipe the cuvette with tissue to remove the fingerprints
  and droplets of liquid.

  Fit the blank cuvette into the 4-cell linear changer and place
  the cuvette in the slot nearest you. For the UNICO UV-2800, push
  the changer so that

  WL : 546.0nm 12: 35: 27

  0.221 AbsD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

• 13

  the cuvette is in the light path (Push the rod in). Close the
  lid. Analyze Sample For different user requirements, we have
  provided different test methods. Basic Mode Push the blank cuvette
  into the light path. In main menu (Fig7),press1 to enter Basic mode
  test . After automatically blanking, it will display as Fig 18
  (automatic changer installed) or Fig 19 ( automatic changer
  uninstalled) and wait for the operator. ESC/STOPto exit.

  Note: .If no automatic changer installed cell #1 andMax E will
  disappear in Fig18

  WL : 656.1nm 12: 35: 27

  0.000 AbsD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  Fig18

  WL : 656.1nm 12: 35: 27

  0.000 AbsD2W

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  Fig19 J Test

  There are three modes (T%,Abs,conc/factor) for you to select by
  pressing F2to make choice.

  WL : 656.1nm 12: 35: 27

  0.104 AbsD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  Fig 20

• 14

  1. Abs mode Push the blank cuvette into the light path.Press F2
  to select Abs mode ,Press 0Abs/100%Tfor Blanking , and then Push
  the sample into lightpath to take reading(Fig 20) 2. T% mode The
  operation is the same as Abs test mode but pressingF2 to select T%
  mode . 3. Conc/Factor mode

  Press F1 to select a concentration unit (Fig 21). If no unit is
  suitable for your test, please select the item Other, press enter
  and input a new unit by pressing the numeric keypad (Fig 22).

  WL : 656.1nm 12: 35: 27

  D2WCell #1

  Max E

  Please select unit: %

  0.000 mg/ml

  F factor 2.000

  Fig 21

  WL : 656.1nm 12: 35: 27

  D2WCell #1

  Max E

  Please input self defined unit: c

  0.000 mg/ml

  F factor 2.000

  Fig 22

  4. Push the blank cuvette into the light path and press
  0Abs/100%Tfor

  Blanking. There are now two choices for you to take: 4.1
  PressF3to input known F value, Fig 23. Then push the sample
  into

  lightpath to take reading of concentration 4.2 Push sample of
  known concentration into the lightpath

  PressF4to input known Conc value, Fig 24. Then push the sample
  into lightpath to take reading of concentration. Note:1.You can
  select wavelength at any time by pressingSET. After your selection,
  instrument always blanks automatically.

  2.If F value is more than 9999,the out of range will display on
  screen.

• 15

  WL : 656.1nm 12: 35: 27

  D2WCell #1

  Max E

  Please input F factor: 4

  0.000 mg/ml

  Fig 23

  WL : 656.1nm 12: 35: 27

  0.000 mg/mlD2WCell #1

  Max E

  F1:Unit F2:Mode F3:F Factor F4:Standard

  F=4.000

  Fig 24

  J Print Test Report

  Press PRINTto print test results (Fig 25).

  Fig 25

  Quantitative Press2in Main Menu for Quantitative Test (Fig 26).
  PressESC/STOPto exit.

  Note: .If no automatic changer installed cell #1 will disappear
  in Fig26. WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Search

  F1:Unit F2:Standard Curve

  Quantitative TestID Abs Conc. (mg/L)

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  Scroll

  Fig 26

• 16

  J How to operation 1. Press F1to select unit of concentration
  (Fig 27).

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Please select unit: g/L

  Quantitative TestID Abs Conc. (mg/L)

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  SearchScroll

  Fig 27

  2 Press SET to select correction methods and enter the

  wavelength. There are three correction methods (single,
  Isoabsorbance and 3 point, Fig 28) . Note: Please refer to the
  Appendix C for the correction method.

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Quantitative TestID Abs Conc. (mg/L)

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  Correction method: Single WL

  SearchScroll

  Fig 28

  3.Press F2in Fig 26 for more items to select .See Fig 29.

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Calibration tableNo Conc. (mg/L) Abs

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  F1:Method F2: Params F3: Standard F4: Curve

  Fig 29 3.1 Press F1in Fig 29 to select fitting method. There are
  4 methods for

  you to choose: Linear fit, linear fit through zero, square fit
  and cubic fit. 3.2 Press F2in Fig 29 to enter directly a known
  standard curve.Fig29A.

• 17

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Calibration tableNo Conc. (mg/L) Abs

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  Input K1=1.033

  Fig 29A The constants to be entered are depending on which
  fitting method selected.The table below lists the their
  relation:

  Fitting Method Fitting Equation constants linear fit through
  zero C=K1A K1, r* Linear fit C=K0+K1A K0,K1,r* square fit
  C=K0+K1A+K2A2 K0,K1,K2 cubic fit C=K0+K1A+K2A2+K3A3 K0,K1,K2,K3

  * r : regression co-efficients, default=1 3.3 Press F3in Fig 29
  to establish a standard curve by measuring a

  group of standard samples. See Fig 30.

  3.3.1 Enter standard concentrations of samples by pressing the
  Numeric keypad followed byENTER. Press or to modify the inputted
  data Fig31. PressESC/STOPto finish inputting and to exit Fig
  32.

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Setup Standard Conc.

  C=1.000*A^1 r=1.000

  Edit the number . . .

  No Conc. (mg/L) Abs 1 0.000 2 -more-

  WL(nm) 700.0

  Fig 30

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  Setup Standard Conc.

  C=1.000*A^1 r=1.000

  Input Standard Conc: 3

  No Conc. (mg/L) Abs 1 2.000 2 3.000 3 -more-

  WL(nm) 700.0

  Fig 31

• 18

  3.3.2 Push the blank cuvette into the light path,
  press0Abs/%100T,the instrument will step to the wavelength and
  blank. See Fig 32.

  D2WCell #1

  F1:Method F2:Params F3:Standard F4:Curve

  Calibration table

  C=1.000*A^1 r=1.000

  No Conc. (mg/L) Abs 1 2.000 2 3.000 3 4.000 4 5.000 5 6.000

  WL : 700.0nm Abs: Blank ..

  WL(nm) 700.0

  Fig 32

  3.3.3 Pull the first sample cuvette of known concentration into
  the light path, Press the keySTARTto get values of standard curve
  one by one (Fig 33).

  Note:If auto-cell changer is installed,the vary samples are
  measured by pressingCELLfollowing numbers(1-8)

  and pressingENTERto comfirm. 3.3.4 PressF4to draw the curve. You
  can get a different curve by

  pressingF1 to select a different fitting method. See Fig
  34-Fig37.

  For linear fits, r represent fitting coefficient of linear
  regression .r=1 is best fitting.usually r is very close to 1.

  Note:If there are few standard samples,it is not suitable for
  selecting square fitting,especially cubic fitting,otherwise invalid
  fitting result will be obtained.

  D2WCell #1

  F1:Method F2:Params F3:Standard F4:Curve

  Calibration table

  C=1.000*A^1 r=1.000

  No Conc. (mg/L) Abs 1 2.000 0.247 2 3.000 0.375 3 4.000 0.532 4
  5.000 0.603 5 6.000 0.764

  WL : 656.1nm Abs: 12: 35: 27

  WL(nm) 700.0

  Fig 33

  D2WCell #1

  Press (ESC) to return . . .

  WL : 656.1nm Abs: 12: 35: 27

  -1.0 Abs 4.0

  0

  C

  onc.

  7

  6

  Fig 34 linear through zero fit

• 19

  D2WCell #1

  Press (ESC) to return . . .

  WL : 656.1nm Abs: 12: 35: 27

  -1.0 Abs 4.0

  0

  C

  onc.

  7

  6

  Fig 35 square fit

  D2WCell #1

  Press (ESC) to return . . .

  WL : 656.1nm Abs: 12: 35: 27

  -1.0 Abs 4.0

  0

  C

  onc.

  91

  438

  Fig 36 cubic fit

  D2WCell #1

  Press (ESC) to return . . .

  WL : 656.1nm Abs: 12: 35: 27

  -1.0 Abs 4.0

  0

  C

  onc.

  7

  6

  Fig 37 linear fit

  3.3.5 Press SAVE to save calibration if required 3.3.6
  PressESC/STOPto exit 4.Quantitative Test

  Before test,the standard curve must be obtained.There are three
  ways for you to obtained it (a, b or c).

  a) Standard curve built up and saved in the instrument.

  In Fig 33 pressLoad and then pressor to select the file with
  type ***.fit. At last pressENTERTO comfirm.

  b) Known standard curve, which is not saved in the instrument.
  See 3.2. For Fig 29 enter a known standard curve directly.

  c) Use the standard samples for the test. First the standard
  curve must be established using the method shown in 3.3. Note: All
  sample results must be taken in screen Fig26.

• 20

  4.1 Push the blank cuvette into the light path and press
  0Abs/100%Tfor blanking.

  4.2 Pull the sample cuvette into the light path, press the
  keySTART, the results will be displayed on the screen (Fig 38).

  WL : 700.0nm Abs: 12: 35: 27

  D2WCell #1

  F1:Unit F2:Standard Curve

  Quantitative Test

  WL(nm) 700.0

  C=1.000*A^1 r=1.000

  SearchScroll

  ID Abs Conc. (mg/L) 1 0.062 0.062 2 0.061 0.061 3 0.062 0.0614
  0.061 0.061

  Fig 38

  4.3 If there is more than one sample, repeat step 4.2 for the
  next sample 4.4 Press (SAVE) to save the results and fitting
  parameters

  J Print Test Report Press the keyPRINTto print the test report
  (Fig 39).

  Fig 40

  WL Scan Press3in main menu for WL Scan test (Fig 41). ESC/STOPto
  exit. To load a previous curve, p ressLOADand select a previously
  stored curve (.wav)

• 21

  D2WCell #1

  WL : 656.1nm Abs: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  F1:Setup F2:Mode F3:Search

  0

  %

  T

  1

  20.0

  XScaleYScale

  Fig 41

  J Scan sample

  1. Press F1 to setup, input the start wavelength, and end
  wavelength by pressing the numeric keypad (Fig 42). Note: The
  UV-2800 scans from high to low wavelength. Browse and select the
  items of scan step and scan speed by pressing or.

  D2WCell #1

  WL : 656.1nm Abs: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  Scan from: 680

  0

  %T

  120

  .0

  XScaleYScale

  Fig 42

  Note: Scan step allows the selection of 0.1nm, 0.2nm ,0.5nm,1nm
  ,2nm and 5nm. Scan speed allows the selection of HI, MEDIUM and
  LOW. For survey scan we suggest 5nm, HI. For detailed scan we
  suggest 0.5nm, HI

  2. Press F2to select the test mode, Abs , %T or E (Fig 43).

  D2WCell #1

  WL : 656.1nm Abs: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  0

  %

  T

  1

  20.0

  From: 200.0To: 680.0Step: 1.0nm

  Please select mode: Abs

  XScaleYScale

  Fig 43

  3. Push the blank cuvette into the light path, press 0Abs/100%T
  to scan the base line (Fig 44). Press the key ESC/STOPto stop

• 22

  scanning;

  D2WCell #1

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  Press (ESC) to stop . . .

  WL : 520.0nm Scan to 200.0nm

  0

  %

  T

  1

  20.0

  XScaleYScale

  Fig 44

  4. Pull the sample cuvette into the light path, press STARTto
  scan the

  sample(Fig 45) ESC/STOP to stop scanning. When scan has finished
  the beeper beeps 3 times (Fig 46).

  D2WCell #1

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  Press (ESC) to stop . . .

  WL : 417.0nm %T: 36.73 Scan to 200.0nm

  0

  %

  T

  1

  20.0

  Fig 45

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  F1:Setup F2:Mode F3:Search

  0

  %

  T

  1

  20.0

  Fig 46

  5 If you want to change the scale, p ress or to change x scale
  (Fig 47), input upper limit and lower limit by pressing the numeric
  keypad . To change y scale press or.

  After these inputs the instrument will redraw the curve (Fig
  48).

• 23

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  Min X:300

  0

  %

  T

  1

  20.0

  Fig47

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  300.0 Wavelength (nm) 500.0

  From: 200.0To: 680.0Step: 1.0nm

  XScaleYScale

  F1:Setup F2:Mode F3:Search

  0

  %

  T

  1

  00.0

  Fig 48

  6 Press F3 to search the Abs/%T value of the scan. There are
  two

  ways for you to search (Fig 49).

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  PointPeak

  F1:Set Peak Height

  0

  %

  T

  1

  20.0

  Fig 49

  a) Peak to peak, press F1 to set peak height and input value
  by

  pressing the numeric keypad (Fig 50). Press to search the peak
  from left to right and pressto search from right to left. The value
  of every peak found will be displayed on the screen one at a time
  (Fig 51).

• 24

  D2WCell #1

  WL : 680.0nm %T: 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  Please input peak height: 0.100

  0

  %

  T

  1

  20.0

  PointPeak

  Fig 50

  D2WCell #1

  WL : 452.0nm %T:22.38 12: 35: 27

  200.0 Wavelength (nm) 680.0

  From: 200.0To: 680.0Step: 1.0nm

  F1:Set peak Height

  0

  %

  T

  1

  20.0

  PointPeak

  Fig 51

  b) Point to point, Press to search the point from left to right
  and

  press ) to search from right to left. The search step interval
  is the same as the scan step. The value of every point searched
  will be displayed on the screen.

  J Save Curve Press SAVEto save the curve. Note: Load/Save
  requires the first scan display page Fig. 48. Press ESC if in
  Search to return to the required page J Print Test Report Press
  PRINTto print the curve you have loaded or scanned (Fig 52). Note:
  The report always is printed in Fig 46

• 25

  Fig 52

  Kinetics

  Press4in main menu for Kinetics (Fig 53). ESC/STOPto exit. To
  load a previous kinetics result, press (LOAD) and select a
  previously stored result (.kin)

• 26

  D2WCell #1

  Tim : 60s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0s

  0

  Abs

  3.

  000

  XScaleYScale

  F1:Setup F2:Mode F3:Process F4:Search

  Fig 53 J Test

  1. Press F1 to set Total Time, Delay Time, Time interval, and
  input the value by pressing the numeric keypad (Fig 54).

  D2WCell #1

  Tim : 60s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0s

  0

  Abs

  3.

  000

  XScaleYScale

  Total Time:180

  Fig 54 2. Select the test mode (Abs or %T) by pressingF2(Fig
  55).

  D2WCell #1

  Tim : 60s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0s

  0

  Abs

  3.

  000

  XScaleYScale

  Please select mode:Abs

  Fig 55 3. Set wavelength by pressingSET .Pull the blank cuvette
  into the

  light path, press 0Abs/100%Tfor blanking 4. Pull the sample
  cuvette into the light path, pressSTART to scan the

  sample. After the delay time, the beeper beeps 3 times and time
  -scan starts. At the end of the time-scan, the beeper also beeps 3
  times (Fig 56);

• 27

  D2WCell #1

  Tim : 180s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0s

  0

  Abs

  1.

  000

  XScaleYScale

  F1:Setup F2:Mode F3:Process F4:Search

  Fig 56

  5. Press F3to process the data, and enter Begin Time, End Time
  and Factor (Fig 57) and the value in I.U. will be calculated and
  displayed (Fig 58). The average straight line between the Begin
  Time and End Time will be calculated. The gradient of this line
  gives the rate of change of ?A/min. Note: I.U.=FactorA/min

  D2WCell #1

  Tim : 60s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0s

  0

  Abs

  3.

  000

  XScaleYScale

  Begin Time:0

  Fig 57

  D2WCell #1

  Tim : 180s Abs: 12: 35: 27

  0 Time(s) 180

  Total T 180s Inteval 1.0sI.U.= +0.364

  0

  Abs

  1.

  000

  XScaleYScale

  F1:Setup F2:Mode F3:Process F4:Search

  Fig 58

  6. If you want to change the scale, please refer to step 5 of WL
  scan. 7. PressF4to search the Abs/%T value in relation to the time
  axis.

  Search point to point by pressing the keyor. Please refer to
  step 6 of WL scan.

• 28

  J Save Curve Press the key SAVE to save curve. Note: Load/Save
  requires the first kinetics display page Fig. 56. Press ESC if in
  Search to return to the required page. J Print Test Report Press
  the keyPRINTto print the curve you have loaded or scanned (Fig
  59).

  Fig 59 DNA/Protein Press5in main menu for DNA/Protein (Fig 60).
  ESC/STOPto exit. Note:The algorithm of the test refer to Appendix A
  please.

• 29

  D2WCell #1

  F1:Coeff F2:Mode F3:Unit F4:Default

  DNA/Protein measurementNo Items Result Unit

  WL : 900.0nm 12: 35: 27

  WL(nm) 260.0 280.0 320.0

  SearchScroll

  Fig 60

  To load previous DNA results, press (LOAD) and select a
  previously stored result (.dna) J Test

  1. To use a simpler or different algorithm, you can enter your
  own values for f1-f4. PressF1to set f1-f4. Input the value by
  pressing the numeric keypad (Fig 61).

  D2WCell #1

  Input f1=62.90

  DNA/Protein measurementNo Items Result Unit

  WL : 900.0nm 12: 35: 27

  WL(nm) 260.0 280.0 320.0

  SearchScroll

  Fig 61

  2. PressF2to select test mode. Absorbance difference 1 is
  for

  testing at the wavelength 260nm,280nm and 320nm (optional),and
  the Absorbance difference 2 is for testing at the wavelength
  260nm,280nm and 320nm (optional,Fig 62). Then select with/without
  reference. If selected with reference (no), the A ref. will be 0
  (Fig 63).

  D2WCell #1

  Measument: Absorbance difference 1

  DNA/Protein measurementNo Items Result Unit

  WL : 900.0nm 12: 35: 27

  WL(nm) 260.0 280.0

  SearchScroll

  Fig 62

• 30

  D2WCell #1

  With reference: Yes

  DNA/Protein measurementNo Items Result Unit

  WL : 900.0nm 12: 35: 27

  WL(nm) 260.0 280.0

  SearchScroll

  Fig 63

  3. Press F3to select the unit of concentration (Fig 64).

  D2WCell #1

  Please select unit: mg/mL

  DNA/Protein measurementNo Items Result Unit

  WL : 900.0nm 12: 35: 27

  WL(nm) 260.0 280.0

  SearchScroll

  Fig 64

  4. Push the blank cuvette into the light path, then press
  0Abs/100%T

  for blanking .

  5. Pull the sample cuvette into the light path, press START to
  test the sample. The test result will be displayed on the screen
  (Fig 65).

  D2WCell #1

  F1:Coeff F2:Mode F3:Unit F4:Default

  DNA/Protein measurementNo Items Result Unit 1 A1 2.947 Abs A2
  2.842 Abs Aref 0.638 Abs

  C-DNA 65.91 mg/mL C-Pro 1672 mg/mL Ratio 1.048

  WL : 900.0nm Abs: 12: 35: 27

  WL(nm) 260.0 280.0 320.0

  SearchScroll

  Fig 65

  6. If there is more than one sample, repeat step 5 for the next
  sample.

  7. Press the key )orfor searching. Input the sample number

  (Fig 66), the result will be displayed on the screen. Press the
  key orto browse the test results one by one.

• 31

  D2WCell #1

  DNA/Protein measurementNo Items Result Unit 1 A1 2.947 Abs A2
  2.842 Abs Aref 0.638 Abs

  C-DNA 65.91 mg/mL C-Pro 1672 mg/mL Ratio 1.048

  WL : 900.0nm Abs: 12: 35: 27

  WL(nm) 260.0 280.0 320.0

  SearchScroll

  Search sample:3

  Fig 66 J Recall the default Press the keyF4to recall the default
  of the f1-f4. J Save Data Press the key SAVE to save data. J Print
  Test Report Press the keyPRINTto print the test result (Fig
  67).

  Fig 67

  Multi Wavelength Press6in main menu for Multi WL (Fig 68).
  ESC/STOPto exit.

  D2WCell #1

  F1:WL setup F2:Mode

  Multi Wavelength TestNo WL(nm) Abs

  500.0

  WL : 900.0nm Abs: 12: 35: 27

  1 WL

  SearchScroll

  Fig 68

  To load previous Multi Wavelength results, press (LOAD) and
  select previously stored results (.mul)

• 32

  J Test 1. Press F1to setup a group of wavelengths for testing by
  pressing the

  numeric keypad followed byENTER. ( ) or (` ) to modify the
  inputted data Fig. 69. PressESC/STOPto finish setup and exit. Note:
  It is recommended to enter the highest wavelength first.

  D2WCell #1

  Multi Wavelength TestWL : 900.0nm Abs: 12: 35: 27

  2 WL

  SearchScroll

  Please input wl:500

  No WL(nm) Abs500.0400.0546.0

  Fig 69

  2. Press F2to select mode (Fig 70).

  D2WCell #1

  Multi Wavelength TestWL : 900.0nm Abs: 12: 35: 27

  3 WL

  SearchScroll

  No WL(nm) Abs500.0400.0300.0

  Please select mode: Abs

  Fig70

  3. Push the blank cuvette into the light path, then press
  0Abs/100%Tfor Blanking .

  4. Pull the sample cuvette into the light path, pressSTART to
  test.

  The test results will be displayed on the screen (Fig 71).

• 33

  D2WCell #1

  F1:WL setup F2:Mode

  Multi Wavelength TestNo WL(nm) Abs 1 500.0 0.87

  400.0 0.42300.0 0.81

  WL : 500.0nm Abs: 12: 35: 27

  3 WL

  SearchScroll

  Fig 71

  5. If there is more than one sample, repeat step 4 for the next
  sample.

  Note: When the test has finished, the wavelength will go to the
  first WL.

  6. Press )orfor searching. Input the sample number, the
  result

  will be displayed on the screen. Press or to browse the test
  results one by one.

  J Save Data Press SAVE to save data. J Print Test Report
  PressPRINTto print the test results (Fig 72).

  Fig 72

  Setting and Calibration Utility Press7in Main menu for Utility
  (Fig 73). ESC/STOPto exit.

• 34

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  123

  98

  4

  67

  WL Reset Printer Lamp Clock

  Accu Validity WL Validity Connect to PC Beeper on/off

  United Products & Instruments INC.

  5 Dark current

  F1 Delete entire saved filesF2 Restore default

  Fig 73

  J WL Reset Press1to reset wavelength (Fig74).

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  123

  98

  4

  67

  WL Reset Printer Lamp Clock

  Accu Vaidity WL Vaidity Connect to PC Beeper on/off

  United Products & Instruments INC.

  5 Dark current

  F1 Delete entire saved filesF2 Restore default

  WL : 482.0nm Step to 900.0nm . . .

  Fig 74

  J Printer Press2to set printer (Fig 75). ESC/STOPto exit.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset printer Select print port Select printer Print report

  United Products & Instruments INC.

  Fig 75 1. Press1in Fig 75 to Reset Printer. 2. Press2in Fig 75
  to select print port (LPT or Comm., Fig 76).

• 35

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset printer Select print port Select printer Print report

  Select the print port: LPT

  Fig 76 3. Press3 in Fig 75 to select printer (HP PCL (1 colour
  cartridge), PCL

  (black mode), Epson ESC/P or Epson/P2 or above, Fig77).

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset printer Select print port Select printer Print report

  Printer : HP PCL (1 color cartridge)

  Fig 77 4. Press4in Fig 75 to select print mode. If you select
  Print screen mode,

  a little icon will be displayed on the top line of the screen
  (Fig 78), if you select Print report mode, the little icon will
  disappear.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset printer Select print port Select printer Print screen

  United Products & Instruments INC.

  Fig78 J Lamp Press3to set lamp (Fig 79). ESC/STOPto exit.

• 36

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset D2 lamp usage time Switch W:

  United Products & Instruments INC.

  Reset W lamp usage time5 Switch point

  Switch D2: ON

  ON

  Fig 79

  1. Press1in Fig 79 to switch on/off D2. Fig 80.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset D2 lamp usage time Switch W:

  United Products & Instruments INC.

  Reset W lamp usage time5 Switch point

  Switch D2: OFF

  OFF

  Fig 80

  2. Press2in Fig 79 to reset usage time of D2(Fig 81). Press
  or

  to select Yes or No, and then press ENTER.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Switch D2: Reset D2 lamp usage time Switch W:

  5 hrs used.Are you sure?

  Reset W lamp usage time5 Switch point

  ON

  ON

  NO

  Fig 81

  3. Press3in Fig 79 to switch on/off W. The indication is also on
  the top

  right corner of the screen (Fig 82).

• 37

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Reset D2 lamp usage time Switch W:

  United Products & Instruments INC.

  Reset W lamp usage time5 Switch point

  Switch D2: ON

  OFF

  Fig 82

  4. Press 4in Fig 79 to reset usage of W (Fig 83). Press or

  to select Yes or No, and then press ENTER.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  D2WCell #1

  1234

  Switch D2: Reset D2 lamp usage time Switch W:

  5 hrs used.Are you sure?

  Reset W lamp usage time5 Switch point

  ON

  ON

  NO

  Fig 83

  5. Press5 in Fig 79 to set the switch usage point of D2 and W
  lamp (Fig

  84).

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST1234

  Switch on/off D2 Reset D2 lamp usage time Switch on/off W

  Input the D2/W switch point:340.0

  Reset W lamp usage time5 Switch point

  D2WCell #1

  84

  J Clock Press 4In Fig73 to set the display mode and modify the
  clock (Fig 85). ESC/STOPto exit.

• 38

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST1234

  Set Time Set Date Show Time mode

  United Products & Instruments INC.

  Show Date mode

  D2WCell #1

  Fig 85

  1. Press 1in Fig 85 to modify time by pressing the numeric
  keypad (Fig

  86).

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST1234

  Set Time Set Date Show Time mode

  Please input the time:08.04.35

  Show Date mode

  D2WCell #1

  Fig 86

  2. Press 2in Fig 85 to modify date by pressing the numeric
  keypad. 3. Press3in Fig 85 to set the date display on the top right
  corner of the

  screen. 4. Press4in Fig 85 to set the time display on the top
  right corner of the

  screen (Fig 87).

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST1234

  Set Time Set Date Show Time mode

  United Products & Instruments INC.

  Show Date mode

  D2WCell #1

  Fig 87

  J Dark Current Press 5In Fig73 to get dark current (Fig 88).

• 39

  SPECTRO-QUEST

  D2WCell #1

  123

  98

  4

  67

  WL Reset Printer Lamp Clock

  Accu Validity WL Validity Connect to PC Beeper on/off

  United Products & Instruments INC.

  5 Dark current

  F1 Delete entire saved filesF2 Restore default

  WL : 656.1nm Get dark current

  UNICO SPECTROPHOTOMETER

  Fig 88

  J Accu Validity Press 4In Fig73 to do accu validity (Fig 89).
  ESC/STOPto exit.

  D2WCell #1

  Photometric Validity TestNo WL(nm) Abs(Std) Abs Result

  WL : 900.0nm Abs: 12: 35: 27

  F1: Standard F2: Mode F3: Tolerance

  Fig 89 1. Press SET to set the wavelength. Press ENTERto edit
  and

  input wavelength by pressing the numeric keypad (Fig 90).
  ESC/STOPto finish inputting and exit.

  D2WCell #1

  Photometric Validity TestWL : 900.0nm Abs: 12: 35: 27

  Please input wl:546

  No WL(nm) Abs(Std) Abs Result 1 440.0

  2 546.0 3 -more-

  Fig 90

  2. PressF1to set the standard value, Press ENTERto edit and
  input

  by pressing the numeric keypad (Fig 91). ESC/STOPto finish
  inputting and exit.

• 40

  D2WCell #1

  Photometric Validity TestWL : 900.0nm %T: 12: 35: 27

  Input the standard:0

  No WL(nm) %T(Std) %T Result 1 440.0 9.28 2 546.0 0.000 3 635.0
  0.000

  Select

  Fig 91

  3. Press F2to select test mode (Abs or %T, Fig 92).

  D2WCell #1

  Photometric Validity TestWL : 900.0nm %T: 12: 35: 27

  No WL(nm) %T(Std) %T Result 1 440.0 9.28 2 546.0 8.45 3 635.0
  5.66

  Please select mode: %T

  Fig 92 4. Press F3to set tolerance (Fig 93).Input the value by
  pressing the

  numeric keypad.

  D2WCell #1

  Photometric Validity TestWL : 900.0nm %T: 12: 35: 27

  No WL(nm) %T(Std) %T Result 1 500.0 9.28 2 600.0 8.45 3 700.0
  5.66

  Input tolerance:0.005

  Fig 93

  5. Press0Abs/100%Tfor Blanking. 6. Put the sample (calibrated
  neutral density filter) into the light path. Press

  STARTto check. The results will be displayed on the screen (Fig
  94). If the discrepancy between the results and the calibrated
  standards is not more than the tolerance, pass will be displayed
  after the test result. Otherwise, fail will be displayed.

  7. The result can be saved,loaded and printed by pressingSAVE,
  LOADand PRINT.

• 41

  D2WCell #1

  Photometric Validity TestWL : 900.0nm %T: 12: 35: 27

  No WL(nm) %T(Std) %T Result 1 500.0 9.28 9.28 pass 2 600.0 8.45
  8.45 pass 3 700.0 5.66

  F1: Standard F2: Mode F3: Tolerance

  Fig 94 J WL Validity Press7in Fig 73 to WL validity (Fig 95).
  ESC/STOPto exit.

  D2WCell #1

  Wavelength Validity TestWL : 900.0nm Abs: 12: 35: 27

  No WL(nm) Peak(nm) T% Result

  F1: Set peaks F2: Mode F3: Tolerance

  Fig 95

  1. Press F1to set the standard peak. Press ENTERto edit and
  input wavelength by pressing the numeric keypad (Fig96). ESC/STOPto
  finish inputting and exit.

  D2WCell #1

  Wavelength Validity TestWL : 900.0nm %T: 12: 35: 27

  Input the standard:0

  No WL(nm) Peak(nm) %T Result 1 241.4 2 361.0 3 417.0 4 537.6 5
  641.4 6 807.4

  Select

  Fig 96

  2. Press F2to select test mode (Abs or %T, Fig 97).

• 42

  D2WCell #1

  WL : 900.0nm %T: 12: 35: 27

  Please select mode: %T

  Wavelength Validity TestNo WL(nm) Peak(nm) %T Result 1 241.4 2
  361.0 3 417.0 4 537.6 5 641.4 6 807.4

  Fig 97

  3. Press F3 to set tolerance (Fig 98). Input the value by
  pressing the

  numeric keypad.

  D2WCell #1

  Wavelength Validity TestWL : 900.0nm %T: 12: 35: 27

  Input tolerance:0.8

  No WL(nm) Peak(nm) %T Result 1 241.4 2 361.0 3 417.0 4 537.6 5
  641.4 6 807.4

  Select

  Fig 98

  4. Press 0Abs/100%Tfor blanking.

  5. Put the sample (calibrated holmium liquid) into the light
  path. Press START to check. The results will be displayed on the
  screen (Fig 99). If the discrepancy between the results and the
  calibrated values is not more than the tolerance, pass will be
  displayed after the test results. Otherwise, fail will be
  displayed.

  D2WCell #1

  WL : 900.0nm %T: 12: 35: 27

  F1: Standard F2: Mode F3: Tolerance

  Wavelength Validity TestNo WL(nm) Peak(nm) %T Result 1 241.4
  239.7 40.06 pass 2 361.0 360.4 42.82 pass 3 417.0 416.9 37.63 pass
  4 537.6 537.2 16.50 pass 5 641.4 641.3 24.33 pass 6 807.4 807.7
  92.38 pass

  Fig 99

  6.The result can be saved,loaded and printed by
  pressingSAVE,LOADand PRINT

  J Connect to PC Press8in Fig 73 to connect to PC (Fig 100), if
  the instrument is on-line with

• 43

  the PC.The screen displays as Fig 100A. PressESC/STOPto
  exit.

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  SPECTRO-QUEST

  United Products & Instruments INC.

  D2W

  Connecting to computer. . .

  Fig 100

  WL : 656.1nm 08: 04: 35

  UNICO SPECTROPHOTOMETER

  United Products & Instruments INC.

  D2W

  Controlled by PC . . .

  SPECTRO-QUEST

  Fig 100A

  J Beeper on/off Press9in Fig 73 to turn on/off the beeper J
  Delete entire saved files PressF1in Fig 73 to delete entire saved
  files. After the delete the files, double confirm need to do. J
  Restore default PressF2in Fig 73 to restore the default parameters.
  Defined test ( auto-cell changer required) Press8in main menu for
  defined test (Fig 101). ESC/STOPto exit.

  D2WCell #1

  F1: Methord F2:Mode

  1 Ref, 1 SampleNo. Abs1

  WL : 900.0nm 12: 35: 27

  0.000 Abs

  Fig 101

• 44

  1. Press F1 to setup method (Fig 102). There are 8 items (1 ref.
  1 sample, 1 ref. 2 samples, 1 ref. 3 samples, 1 ref. 4 samples, 1
  ref. 5 samples, 1 ref. 6 samples, 1 ref. 7 samples, N refs. N
  samples) for selecting. We take 1 ref. 4 samples for example,Fig
  102

  D2WCell #1

  Select define test: 1 Ref.,4 samples

  1 Ref, 4 SamplesNo. Abs1234

  WL : 900.0nm 12: 35: 27

  0.000 Abs

  Fig 102

  2. Press F2to select test mode (Abs or %T, Fig 103).

  D2W

  Please select mode: Abs

  4 Ref, 4 SampleNo. Abs1234

  WL : 900.0nm 12: 35: 27

  0.000 Abs

  Fig 103

  3. Put the reference into cell NO.1 and 4 samples into cell NO.2
  -NO.5.Set

  wavelength. 4. Press START.Automatically the reference is taken
  in cell NO.1,the 4

  samples are taken in cell NO.2-NO.5. The results are displayed
  as Fig 104.

  D2WCell #1

  1 Ref, 1 SampleNo. Abs1 0.2272 0.2893 0.3384 1.106

  0.000 Abs

  F1: Methord F2:Mode

  WL : 900.0nm 12: 35: 27

  Fig 104

  5. Select N refs. N samples,Take 8 refs. 8 samples for example.
  6. After setup wavelength and mode(%T or Abs),put 8 references into
  CELL

  NO.1-NO.8.

• 45

  7. PressSTART,the screen display as Fig105,thePlace 1st group
  appear on the right of top line, Press0Abs/100%T, the 8 references
  are taken automatically and the screen change to Fig 106.

  D2WCell #1

  1 Ref, 1 SampleNo. Abs12345678

  0.000 Abs

  F1: Methord F2:Mode

  WL : 900.0nm Place 1st group . .

  Fig 105

  D2WCell #1

  1 Ref, 1 SampleNo. Abs12345678

  0.000 Abs

  F1: Methord F2:Mode

  WL : 900.0nm Place 2nd group . .

  Fig 106

  8. Remove 8 references and put 8 samples into CELL
  NO.1-NO.8,Press the START,the results are taken automatically . Fig
  107.

  D2WCell #1

  1 Ref, 1 SampleNo. Abs1 0.1272 0.1893 0.2384 0.8065 1.4426
  1.5677 1.6698 1.741

  0.000 Abs

  F1: Methord F2:Mode

  WL : 900.0nm 12: 45: 57

  Fig 107

• 46

  Appendix A

  DNA/Protein Test Algorithm Test Name Method Wavelength(s)
  Calculations Parameters Displayed

  Units DNA MEASUREMENT

  Absorbance difference (260,280)

  A1=A260nm A2=A280nm Aref=A320nm (optional)

  DNA concentration: (A1-Aref)f1-(A2-Aref)f2 Protein concentration
  (A2-Aref)f3-(A1-Aref)f4

  f1=62.9 f2=36.0 f3=1552 f4=757.3

  Absorbance difference (260,230)

  A1=A260nm A2=A230nm Aref=A320nm (optional)

  DNA concentration: (A1-Aref)f1-(A2-Aref)f2 Protein concentration
  (A2-Aref)f3-(A1-Aref)f4

  f1=49.1 f2=3.48 f3=183 f4=75.8

  DNA: g/ml Protein:g/ml

  DNA/Protein Concentration and DNA purity

  Absorbance ratio

  A1=A260nm A2=A280nm or A230nm Aref=A320nm (optional)

  Ratio= A1-Aref

  A2-Aref

  None No units(ratio)

• 47

  Appendix B Lamp Replacement A. TO REPLACE DEUTERIUM LAMP 1. Turn
  off and unplug the instrument (VERY IMPORTANT: HIGH

  VOLTAGE). 2. Remove the cuvette holder rod by unscrewing the
  rod

  counterclockwise. 3. Remove the all screws around the sides of
  the spectrophotometer.

  See Fig A1

  Fig A1 4. Very carefully remove the cover of the instrument and
  place in right

  side of the instrument. Fig A2

• 48

  Fig A2 HINT: If it is necessary to remove the cover from the
  right side of the instrument, carefully remove 3 connectors (CZ6,
  CZ4 and J3)on PCB marked SST8.417.100 . Be sure to reconnect after
  replacing the lamp! Fig A3

  CZ6 Fig A3

  5. Remove the grey metal protection cover. Using screwdrivers
  remove the two top screws and the two bottom screws, and then

  J3

  CZ4

• 49

  place the protective cover to the side. See Fig A4

  Fig A4

  6. Disconnecting the connector J7 on the PCB marked
  SST8.411.128. Unscrew the screw that holds the lamp bracket to the
  instrument base. Pull the entire lamp and lamp holder assembly out.
  See Fig A5

• 50

  J7

  Fig A5 7. Replace the pre-aligned lamp with a lamp (Fig A6)
  provided by

  UNICOor an authorized UNICOService Provider . This comes
  pre-assembled with lamp socket.

  Fig A6 CAUTION: THE LAMP MAY BE HOT! TAKE PRECAUTIONS TO PREVENT
  POSSIBLE BURNS.

  8. Reconnect the connector J7 to the PCB marked
  SST8.411.128.

• 51

  9. Re-fit the grey metal protection cover, Fig. A4. Temporarily
  re-fit the main cover and fix with two screws, one each side.
  Switch on and remove the grommet from the middle of the rear panel.
  You can now look through the hole and view the image of the lamp on
  the slit. Check the lamp alignment Fig. A7. If the image is not
  covering the slit, the lamp alignment needs adjustment. This
  requires running the UV-2800 without the covers, with high voltages
  accessible, and so should only be performed by a suitably qualified
  engineer. If adjustment is required, remove the cover and grey
  protection cover, put on UV protection glasses and turn on the
  instrument. Adjust to make the image central on the slit, Fig. A7.
  Install the grey metal protection cover and cover of
  instrument.

  Fig A7

  CAUTION: Wear UV protection glasses when replacing deuterium
  lamp. 10. Re-fit all the screws around the sides of the
  spectrophotometer, Fig. A1. 11. Re-set the lamp usage time. Select
  Utility, lamp, and re-set D2 usage

  time.

  Focus on the slit

• 52

  B. TO REPLACE TUNGSTEN-HALOGEN LAMP 1. The step 1- step 5 are
  the same as the REPLACING DEUTERIUM. 2. Remove the lamp from the
  ceramic base. 3. Insert the new lamp (Fig A8), pushing it in as far
  as it will go. 4. Re-fit the grey metal protection cover, Fig. A4.
  Temporarily re-fit the

  main cover and fix with two screws, one each side. Switch on and
  remove the grommet from the middle of the rear panel. You can now
  look through the hole and view the image of the lamp on the slit.
  Check the lamp alignment Fig. A9. If the image is not covering the
  slit, the lamp alignment needs adjustment. This requires running
  the UV-2800 without the covers, with high voltages accessible, and
  so should only be performed by a suitably qualified engineer. If
  adjustment is required, remove the cover and grey protection cover
  and turn on the instrument. Adjust to make the image central on the
  slit, Fig. A9. Install the grey metal protection cover and
  instrument cover.

  Fig A8

• 53

  Fig A9 CAUTION: DO NOT HANDLE THE LAMP WITH BARE FINGERS. USE
  TISSUE OR CLOTH WHEN HANDLING LAMP. The oil from your fingers can
  cause the lamp to burn out prematurely. 5. Re-fit all the screws
  around the sides of the spectrophotometer, Fig. A1 6. Install the
  gray metal protection cover and cover of instrument. 7. Re-set the
  tungsten lamp usage time. Select Utility, lamp and re-set

  W lamp usage time.

  Focus on the slit

• 54

  Appendix C A number of correction techniques can be used to
  eliminate or reduce interference errors. In general, if the source
  of the error is known and is consistent from sample to sample, the
  error can be eliminated. On the other hand, if the source is
  unknown and varies from sample to sample, the error can be reduced
  but not eliminated. Correction techniques can always require data
  from at least two wavelengths. The more sophisticated correction
  techniques require multiwavelength or spectral data. A.1
  Isoabsorbance When a known interfering component with a known
  spectrum is present, the error introduced by this component at the
  analytical wavelength for the target analyte can be eliminated by
  selecting a reference wavelength at which the interfering compound
  exhibits the same absorbance as it does at the analytical
  wavelength. The absorbance at this reference wavelength is
  subtracted from the absorbance at the analytical wavelength, as
  shown in Figure A1.The residual absorbance is the true absorbance
  of the analyte. This technique is less reliable when the spectra of
  the analyte and of the interferent are highly similar. Moreover, it
  can correct for only one interference

  Fig A1 Isoabsorbance correction

  A.2 Three-point correction The three-point, or Morton-Stubbs
  correction uses two reference

  wavelengths, usually those on either side of the analytical
  wavelength.

• 55

  The background interfering absorbance at the analytical
  wavelength is then estimated using linear interpolation (see Figure
  A2).This method represents an improvement over the
  single-wavelength reference technique because it corrects for any
  background absorbance that exhibits a linear relationship to the
  wavelength. In many cases, if the wavelength range is narrow, it
  will be a reasonable correction for non-linear background
  absorbances such as that resulting from scattering of from a
  complex matrix.

  Fig A2

Однолучевой спектрофотометр ЮНИКО модель 2800, работающий в спектральном диапазоне 190-1100 нм,  является самой экономичной разработкой в серии однолучевых приборов ЮНИКО 280X. Эта модель, имеющая спектральную щель 4 нм и скорость сканирования до 1000 нм/мин специально адаптирована для отечественных условий и выпускается с учетом российских лабораторных требований. Прибор отличается высокой точностью определения процента пропускания/ оптической плотности, установки длины волны, разрешением 0,1 нм и  прекрасной временной стабильностью.

Спектрофотометр ЮНИКО 2800 является настольным прибором, оснащенным большим информативным жидкокристаллическим экраном.(320х240 точек), 29-знаковой клавиатурой, встроенным мощным процессором, позволяющим проводить все аналитические измерения без подключения к персональному компьютеру. Режимы измерений, градуировки и полученные спектры хранятся в энергонезависимой памяти. Для вывода информации предусмотрены порты для подключения принтера и компьютера. Для удобства хранения и обработки данных на персональном компьютере разработано специальное программное обеспечение, обеспечивающее перенос данных, составление отчетов, представление результатов измерений.

Вместительное отделение  для проб позволяет размещать кюветы длиной до 100 мм. Предлагается широкий ряд держателей проб/кювет и дополнительных принадлежностей. Возможна установка кассеты для программируемой автоматической смены 8-ми квадратных кювет 10х10 мм. Для медико-биологических измерений особенно интересна проточная терморегулируемая  (15-40°С) система с собственным перистальтическим насосом и программатором температуры на Пельтье-элементах.

Конструкция спектрофотометров ЮНИКО серии 280Х основана на оригинальных оптических элементах с защитным покрытием, смонтированных на мощной плите. Источниками излучения служат дейтериевая и галогеновая лампы, доступные для самостоятельной смены и юстировки. Программный счетчик времени работы ламп позволяет планировать их эксплуатацию и своевременную замену.

Метрологическая поддержка.

На основании положительных результатов испытаний спектрофотометр ЮНИКО модель 2800 внесен как тип в государственный реестр средств измерений РФ и зарегистрирован под № 38106-08, выдан сертификат Госстандарта РФ №  32007.

Спектрофотометры ЮНИКО 2800 имеют описание типа средств измерений, снабжаются свидетельством о госпроверке, методикой поверки, руководством по эксплуатации на русском языке.

Приборы подлежат ежегодной поверке силами ЦСМ на местах с использованием стандартных поверочных средств.